Class 12 Biology Biotechnology and Its Applications MCQs with Answer

Class 12 Biology Biotechnology and Its Applications MCQs by Sainik School Agra Classes serve as invaluable resources for students navigating the intricate landscape of biotechnology. These MCQs encapsulate the essence of biotechnological concepts, offering a structured approach to comprehension and assessment. With a focus on Class 12 syllabi, these questions delve into genetic engineering, recombinant DNA technology, and applications like gene therapy and genetically modified organisms (GMOs). They serve as diagnostic tools, gauging students’ understanding while fostering critical thinking and problem-solving skills. By integrating theory with practical applications, Sainik School Agra Classes cultivates a holistic understanding of biotechnology, empowering students to navigate the complexities of modern biology and contribute meaningfully to scientific discourse and innovation.

Q1. The first clinical gene therapy was done for the treatment of:

1. AIDS.
2. Cancer.
3. Cystic fibrosis.
4. SCID (Servere Combined Immuno Deficiency resulting form deficiency of ADA)

Q2. A protoxin is:

1. A primitive toxin.
2. A denatured toxin.
3. Toxin produced by protozoa.
4. Inactive toxin.

Q3. ELISA technique is based on the principle of:

1. DNA replication.
2. Antigen and Antibody interaction.
3. Pathogen and Antigen interaction.
4. Antigen and Protein interaction.

Q4. The nematode that infests and damages tobacco roots:

1. Ascaris megalocephala.
2. Ancylostoma.
3. Meloidegyne incognitia.
4. Trichinella.

Q5. How does proinsulin become mature?

1. By adding a short peptide.
2. By removal of C-peptide.
3. By cutting into 2 halves.
4. By changing its 3D shape.

Q6. The source of alkaline phosphatase enzyme:

1. BAP.
2. CAP.
3. YAC.
4. Both (a) and (b).

Q7. Which among the following is based on antigen antibody interaction?

1. PCR.
2. Electrophoresis.
3. ELISA.
4. All of these.

Q8. The site of production of ADA in the body is:

1. Bone marrow.
2. Lymphocytes.
3. Blood plasma.
4. Monocytes.

Q9. A probe which is a molecule used to locate specific sequences in a mixture of DNA or RNA molecules could be:

1. A single stranded RNA.
2. A single stranded DNA.
3. Either RNA or DNA.
4. Can be ss DNA but not ss RNA.

Q10. The trigger for activation of toxin of Bacillus thuringiensis is:

1. Acidic pH of stomach.
2. High temperature.
3. Alkaline pH of gut.
4. Mechanical action in the insect gut.

Q11. α − 1 antitrypsin is:

1. An antacid.
2. An enzyme.
3. Used to treat arthritis.
4. Used to treat emphysema.

Q12. Golden rice is:

1. A variety of rice grown along the yellow river in China.
2. Long stored rice having yellow colour tint.
3. A transgenic rice having gene for-carotene.
4. Wild variety of rice with yellow coloured grains.

Q13. In RNAi, genes are silenced using:

1. ss DNA.
2. ds DNA.
3. ds RNA.
4. ss RNA

Q14. Bt cotton is not:

1. A GM plant.
2. Insect resistant.
3. A bacterial gene expressing system.
4. Resistant to all pesticides.

Q15. GEAC stands for:

1. Genome Engineering Action Committee.
2. Ground Environment Action Committee.
3. Genetic Engineering Approval Committee.
4. Genetic and Environment Approval committee.

Q16. C-peptide of human insulin is:

1. A part of mature insulin molecule.
2. Responsible for formation of disulphide bridges.
3. Removed during maturation of pro-insulin to insulin.
4. Responsible for its biological activity.

Q17. Nematode specific genes were introduced into the tobacco host plant using a vector:

1. pBR 322.
2. Plasmid.
3. Bacteriophage.
4. Agrobacterium.

Q18. Which among these is true for nucleases?

1. They are nucleic acids.
2. They are proteins.
3. They are molecular glue.
4. They are carbohydrates.

Q19. Which among the following is not allowed to take place in the case of RNA interference employed in making
tobacco plants resistant to the nematode, Meloidegyne incognitia?

1. Transcription of mRNA.
2. Translation of mRNA.
3. Replication of DNA.
4. Maturation of hn RNA.

Q20

. Which among the following is true for BT toxin?

1. It kills the lepidopterans.
2. It is coded by ‘cry’ gene.
3. It exists in bacteria as prototoxin.
4. All are true.