Class 12 Biotechnology Principal and Processes MCQs with Answers

Class 12 Biology Biotechnology Principal and Processes MCQs with Answers

At Sainik School, our Class 12 Biology MCQs on “Biotechnology: Principles and Processes” provide students with a comprehensive platform to master this significant topic. Covering various aspects of biotechnology, including genetic engineering techniques, gene cloning, DNA fingerprinting, and biotechnological applications, these multiple-choice questions offer thorough preparation for Class 12 Biology exams.

Our MCQs challenge students to apply their knowledge of biotechnological principles and processes to solve problems and analyze scenarios, fostering critical thinking and analytical skills essential for success in exams. Each MCQ is accompanied by a detailed answer explanation, enabling students to grasp the underlying concepts thoroughly.

By practicing Class 12 Biology MCQs on “Biotechnology: Principles and Processes” at Sainik School, students not only prepare effectively for their exams but also gain a deeper understanding of the applications and implications of biotechnology in various fields, laying a strong foundation for their future studies and careers in biology and related fields.

Q1. In genetic engineering experiments, restriction enzymes are used for:

  1. Viral DNA.
  2. Bacterial DNA.
  3. Eukaryotic DNA.
  4. Any type of DNA.
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Ans: 2. Bacterial DNA.

Q2. A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the
transformed cells did not produce the desired protein. Reasons could be:

  1. Human gene may have intron which bacteria cannot process.
  2. Amino acid codons for humans and bacteria are different.
  3. Human protein is formed but degraded by bacteria.
  4. All of the above.
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Ans: 1. Human gene may have intron which bacteria cannot process.

Any nucleotide sequence in a gene that is removed by RNA splicing during maturation of final RNA product is called intron. Intron can
be different in different organisms.

Q3. Two statements are given-one labelled Assertion and the other labelled Reason. Select the correct answer to 1 Mark
these questions from the codes (a), (b), (c) and (d) as given below.

  1. Both assertion and reason are true and reason is the correct explanation of assertion.
  2. Both assertion and reason are true but reason is not the correct explanation of assertion.
  3. Assertion is true but reason is false.
  4. Both assertion and reason are false.
    Assertion: cDNA is copy DNA which is synthesised in vivo on a DNA template using DNA polymerase.
    Reason: cDNA of all possible genes are ti gated with different plasmids and maintained in either plant or animal
    cells.
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Ans: 4. Both assertion and reason are false.

Q4. Who among the following was awarded the Nobel Prize for the development of PCR technique?

  1. Herbert Boyer.
  2. Hargovind Khurana.
  3. Kary Mullis.
  4. Arthur Kornberg.
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Ans: 3. Kary Mullis.
Kary Mullis was awarded the Nobel Prize for the development of PCR technique.

Q5. Which of the following has popularised the PCR (polymerase chain reactions)?

  1. Easy availability of DNA template.
  2. Availability of synthetic primers.
  3. Availability of cheap deoxyribonucleotides.
  4. Availability of ‘Thermostable’ DNA polymerase.
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Ans: 4. Availability of ‘Thermostable’ DNA polymerase.

Q6. In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host
cells are then cultured on a medium containing ampicillin. The result will be:

  1. Both transformants and non-transformants cannot survive.
  2. Both transformants and non-transformants can survive.
  3. Transformants only and not the non transformants can survive.
  4. Transformants cannot survive, but non transformants can not.
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Ans: 3. Transformants only and not the non transformants can survive.

Q7. Which one of the following is related with genetic engineering?

  1. Plastids.
  2. Mitochondria.
  3. Plasmids.
  4. Lysosomes.
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Ans: 3. Plasmids

Q8. The first isolated restriction endonuclease is:

  1. Hind II.
  2. Hind III.
  3. EcoRI.
  4. Alu I.
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Ans: 1. Hind II.

Q9. The transfer of genetic material from one bacterium to another through the mediation of a vector like virus is
termed as:

  1. Transduction.
  2. Conjugation.
  3. Transformation.
  4. Translation
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Ans: 1. Transduction.

Q10. Which is a direct gene transfer method?

  1. Using Cosmid.
  2. Using Agrobacterium.
  3. Using BAC.
  4. Electroporation
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Ans: 4. Electroporation.

Q11. Which of the given statement is correct in the context of observing DNA separated by agarose gel
electrophoresis?

  1. DNA can be seen in visible light.
  2. DNA can be seen without staining in visible light.
  3. Ethidium bromide stained DNA can be seen in visible light.
  4. Ethidium bromide stained DNA can be seen under exposure to UV light.
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Ans: 4. Ethidium bromide stained DNA can be seen under exposure to UV light.

Q12. In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

  1. Charge only.
  2. Size only.
  3. Charge to size ratio.
  4. All of the above.
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Ans: 4. All of the above.
The differential mobility of DNA depends upon charge and size of DNA.

Q13. A recombinant DNA molecule can be produced in the absence of the following:

  1. Restriction endonuclease.
  2. DNA ligase.
  3. DNA fragments.
  4. E.coli.
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Ans: 4. E.coli.

Q14. The DNA fragments produced by the use of restriction endonucleases can be separated by:

  1. Polymerase chain reaction.
  2. Gel electrophoresis.
  3. Density gradient centrifugation.
  4. Any of the above.
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Ans: 2. Gel electrophoresis.

Q15. ‘Restriction’ in Restriction enzyme refers to:

  1. Cleaving of phosphodiester bond in DNA by the enzyme.
  2. Cutting of DNA at specific position only.
  3. Prevention of the multiplication of bacteriophage in bacteria.
  4. All of the above.
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Ans: 3. Prevention of the multiplication of bacteriophage in bacteria.

FAQs

How do MCQs on “Biotechnology: Principles and Processes” in Class 12 Biology help students understand the fundamentals of genetic engineering and biotechnological techniques?

MCQs on “Biotechnology: Principles and Processes” play a crucial role in aiding Class 12 Biology students to comprehend the fundamentals of genetic engineering and various biotechnological techniques. These MCQs cover topics such as gene cloning, DNA fingerprinting, polymerase chain reaction (PCR), and recombinant DNA technology. By engaging with these MCQs, students can deepen their understanding of the principles underlying these techniques, enabling them to analyze and solve problems related to biotechnology effectively.

What strategies can students employ to effectively prepare for Class 12 Biology exams using MCQs on “Biotechnology: Principles and Processes”?

To effectively prepare for exams using MCQs on “Biotechnology: Principles and Processes,” students should first ensure a solid understanding of the fundamental concepts and techniques within biotechnology. They can then practice solving a variety of MCQs from reputable sources, focusing on different aspects such as gene cloning, DNA manipulation techniques, and biotechnological applications. It’s essential to analyze both correct and incorrect answers to deepen understanding and identify areas for improvement. Additionally, students can use MCQs to simulate exam conditions, helping them become familiar with the format and timing of the actual examination. Regular practice with MCQs, coupled with comprehensive revision of concepts, can significantly enhance students’ performance in Class 12 Biology exams.

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